A genomic approach for isolating chloroplast microsatellite markers for Pachyptera kerere (Bignoniaceae)1. (19th September 2016)

• Record Type: Journal Article
• Authors: Francisco, Jessica N. C.; Nazareno, Alison G.; Lohmann, Lúcia G.
• Is Part Of: Applications in plant sciences. Volume 4:Number 9(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-09-19
• Subjects: Bignoniaceae -- Bignonieae -- chloroplast genome -- microsatellite -- Pachyptera kerere -- transferability; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1600055
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: In this study, we developed chloroplast microsatellite markers (cpSSRs) for Pachyptera kerere (Bignoniaceae) to investigate the population structure and genetic diversity of this species. Methods and Results: We used Illumina HiSeq data to reconstruct the chloroplast genome of P. kerere by a combination of de novo and reference‐guided assembly. We then used the chloroplast genome to develop a set of cpSSRs from intergenic regions. Overall, 24 primer pairs were designed, 21 of which amplified successfully and were polymorphic, presenting three to nine alleles per locus. The unbiased haploid diversity per locus varied from 0.207 (Pac28) to 0.817 (Pac04). All but one locus amplified for all other taxa of Pachyptera . Conclusions: The markers reported here will serve as a basis for studies to assess the genetic structure and phylogeographic history of Pachyptera .