Development of microsatellite markers for Sargentodoxa cuneata (Lardizabalaceae) using next‐generation sequencing technology1. (9th May 2016)

• Record Type: Journal Article
• Authors: Sun, Zhi‐Xia; Ye, Lin‐Jiang; Zhang, Fei; Hu, Wan; Fan, Deng‐Mei; Zhang, Zhi‐Yong
• Is Part Of: Applications in plant sciences. Volume 4:Number 5(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-05-09
• Subjects: genetic diversity -- Illumina MiSeq -- Lardizabalaceae -- microsatellite markers -- Sargentodoxa cuneata; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1600003
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: Microsatellite loci were developed for a woody deciduous liana, Sargentodoxa cuneata (Lardizabalaceae), to help infer the evolutionary histories of ancient monotypic genera in subtropical China. Methods and Results: Using next‐generation sequencing (Illumina MiSeq) technology, 21 polymorphic primer sets were identified in three wild populations. The number of alleles per locus ranged from one to seven. The expected and observed heterozygosities varied from 0 to 0.788 and 0 to 0.917, respectively. Transferability analyses were performed in Stauntonia chinensis, Akebia trifoliata, and A. quinata . Eighteen (85.7%), 18 (85.7%), and 17 (81.0%) markers were successfully amplified, respectively. Conclusions: The newly developed markers will facilitate further studies on genetic diversity and phylogeographic patterns throughout the distributional range of S. cuneata . This set of microsatellite primers represents the second report on molecular markers in Lardizabalaceae.