Characterization of 16 microsatellite markers for the Oreinotinus clade of Viburnum (Adoxaceae)1. (8th December 2016)

• Record Type: Journal Article
• Authors: Barish, Syndi; Arakaki, Mónica; Edwards, Erika J.; Donoghue, Michael J.; Clement, Wendy L.
• Is Part Of: Applications in plant sciences. Volume 4:Number 12(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-12-08
• Subjects: Adoxaceae -- genetic diversity -- Viburnum dentatum -- Viburnum hallii -- Viburnum pichinchense -- Viburnum trilobum -- Viburnum triphyllum; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1600103
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: Microsatellite loci were isolated from four species of Viburnum (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American Viburnum species of the Oreinotinus clade. Methods and Results: Using a microsatellite‐enriched library and mining next‐generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of V. triphyllum and one population of V. pichinchense . For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population. Conclusions: The 16 microsatellite loci developed for the Oreinotinus clade ( Viburnum, Adoxaceae) will inform investigations of population structure and species boundaries within this group.