Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)1. (11th May 2015)

• Record Type: Journal Article
• Authors: Letelier, Luis; Harvey, Nick; Valderrama, Aly; Stoll, Alexandra; González‐Rodríguez, Antonio
• Is Part Of: Applications in plant sciences. Volume 3:Number 5(2015)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2015-05-11
• Subjects: Chile -- microsatellites -- Quillaja saponaria -- Quillajaceae -- soapbark; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1500024
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. Methods and Results: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria . All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations. Conclusions: The microsatellite markers described here are the first characterized for Q. saponaria . The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species.