Development of phylogenetic markers for Sebacina (Sebacinaceae) mycorrhizal fungi associated with Australian orchids1. (4th June 2014)

• Record Type: Journal Article
• Authors: Ruibal, Monica P.; Peakall, Rod; Foret, Sylvain; Linde, Celeste C.
• Is Part Of: Applications in plant sciences. Volume 2:Number 6(2014)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2014-06-04
• Subjects: mycorrhizal fungi -- orchids -- phylogenetics -- Sebacina; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1400015
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: To investigate fungal species identity and diversity in mycorrhizal fungi of order Sebacinales, we developed phylogenetic markers. These new markers will enable future studies investigating species delineation and phylogenetic relationships of the fungal symbionts and facilitate investigations into evolutionary interactions among Sebacina species and their orchid hosts. Methods and Results: We generated partial genome sequences for a Sebacina symbiont originating from Caladenia huegelii with 454 genome sequencing and from three symbionts from Eriochilus dilatatus and one from E. pulchellus using Illumina sequencing. Six nuclear and two mitochondrial loci showed high variability (10–31% parsimony informative sites) for Sebacinales mycorrhizal fungi across four genera of Australian orchids ( Caladenia, Eriochilus, Elythranthera, and Glossodia ). Conclusions: We obtained highly informative DNA markers that will allow investigation of mycorrhizal diversity of Sebacinaceae fungi associated with terrestrial orchids in Australia and worldwide.