Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)1. (31st January 2013)

• Record Type: Journal Article
• Authors: Werth, Silke; Cornejo, Carolina; Scheidegger, Christoph
• Is Part Of: Applications in plant sciences. Volume 1:Number 2(2013)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2013-01-31
• Subjects: Ascomycetes -- Ascomycota -- lichen fungi -- Lobaria pulmonaria -- microsatellites -- population subdivision; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1200290
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross‐amplification with L. immixta and L. macaronesica . The number of alleles per locus ranged from two to 23. Nei's unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria .