First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)1. (31st January 2013)

• Record Type: Journal Article
• Authors: Molinier, Virginie; Murat, Claude; Morin, Emmanuelle; Gollotte, Armelle; Wipf, Daniel; Martin, Francis
• Is Part Of: Applications in plant sciences. Volume 1:Number 2(2013)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2013-01-31
• Subjects: direct shotgun pyrosequencing -- polymorphism -- truffle -- Tuber aestivum -- Tuberaceae; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.3732/apps.1200220
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the study: Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. Methods and Results: Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. Conclusions: Direct shotgun pyrosequencing is a fast and relatively low‐cost technique allowing identification of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species.