Determination of Genotoxic Damage by Comet Assay in Smokers. (3rd April 2017)

• Record Type: Journal Article
• Authors: Karahan, E. G.; Tomatir, A. G.; Acikbas, I.; Er, A. B.; Evyapan, F.; Akdag, B.; Arslan, P. E.
• Is Part Of: International journal of human genetics. Volume 17:Number 2(2017)
• Page Start: 64
• Page End: 71
• Publication Date: 2017-04-03
• Subjects: Genomic Instability -- Genotoxicity -- Peripheral Blood -- Single Cell Gel Electrophoresis -- Smoking; Human genetics -- Periodicals; 599.935
• DOI: 10.1080/09723757.2017.1351121
• Languages: English
• ISSNs: 0972-3757

Abstract

ABSTRACT: The clinical course of most diseases related to smoking has a strong relationship with genotoxicity. In this study, the researchers aimed to compare DNA damage of smokers and non-smokers to determine the genotoxic risk. In total, 50 volunteers were included in this study; 30 of them smokers and 20 of them forming the non-smoker control group. Peripheral blood samples taken from the volunteers were determined with comet assay. The researchers determined the DNA damage ratio as 12, 75 (± 7. 14) from smokers 10, 41 (± 3.41) from non-smokers (p> 0.05), and also higher DNA damage in male smokers than female ones (p<0. 05). There was no correlation between age and DNA damage. In conclusion, there was no significant difference between smokers and non-smokers in terms of DNA damage, but there were some important changes in the DNA of smokers and genomic instability was adversely affected.