Involvement of indirectly allostimulated CD4+CD43highCD45RO+ T cell proliferation in the development of chronic allograft nephropathy. Issue 11 (June 2016)

• Record Type: Journal Article
• Authors: Wee, Yu-Mee; Jung, Joo-Hee; Kim, Yang-Hee; Choi, Monica-Y; Kim, Young-Hoon; Choi, Do-Sook; Cho, Myung-Hwan; Han, Duck-Jong
• Is Part Of: Experimental biology and medicine. Volume 241:Issue 11(2016)
• Page Start: 1217
• Page End: 1228
• Publication Date: 2016-06
• Subjects: CD4+CD43highCD45RO+ T cell -- indirect allostimulation -- effector memory T cells -- induced protein-10 -- chronic allograft nephropathy -- progression of acute rejection; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/1535370215601522
• Languages: English
• ISSNs: 1535-3702

Abstract

The goal of this study was to identify immunological markers for use in antigen-specific assays that predict long-term survival after renal allograft and distinguish stable-functioning (SP) patients from poorly functioning (PP) patients. For this prospective study, 20 patients were enrolled. Eight SP and six PP patients were enrolled in this study. Serum cytokine/chemokine levels were analyzed by the Luminex multiplex assay. To detect indirect alloreactive T cells, we performed indirect mixed lymphocyte reaction using donor-antigen-pulsed autologous dendritic cells as stimulators. Serum induced protein-10 levels were significantly higher in the serum of PP patients, whereas sCD40L levels were higher in SP patients. The PP patients had significantly higher numbers of donor-specific CD4 + CD43 high CD45RO + T cells after indirect allostimulation, whereas this cell population was unchanged in SP patients. The donor-specific CD4 + CD43 high CD45RO + T cells had the effector memory T cell phenotype. Prospectively, we studied whether these cells influence graft outcome and found that their strong proliferation in pre-transplant patients is related to a poorly functioning graft. Indirectly allostimulated CD4 + CD43 high CD45RO + T cells may not only contribute to chronic allograft nephropathy development but may also have a role in the progression of acute rejection. Thus, these cells may have potential use as immune-monitoring markers in a noninvasive in vitro assay that predicts graft outcome.