A highly sensitive and direct competitive enzyme-linked immunosorbent assay for the detection of di-(2-ethylhexyl) phthalate (DEHP) in infant supplies. Issue 13 (5th June 2015)

• Record Type: Journal Article
• Authors: Zhang, Mingcui; Hong, Wentong; Wu, Xiayu; Zhang, Yue; Li, Fengzhu; Zhao, Su-Qing
• Is Part Of: Analytical methods. Volume 7:Issue 13(2015)
• Page Start: 5441
• Page End: 5446
• Publication Date: 2015-06-05
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay00207a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A sensitive and specific direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was studied in this paper for the detection of di-(2-ethylhexyl) phthalate (DEHP) based on an antigen-coating format. Abstract : A sensitive and specific direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was studied in this paper for the detection of di-(2-ethylhexyl) phthalate (DEHP) based on an antigen-coating format. The DEHP-specific polyclonal antibody was raised in rabbits and used to construct the dc-ELISA for the measurement of DEHP. The conjugates of the antibody with horseradish peroxidase (HRP) were used as the detection probe. Under optimal conditions, the assay had a detection limit (LOD) of about 0.0042 ng mL −1, with an apparent linear range of 10 −3 −10 3 ng mL −1 ( R 2 = 0.998). The cross-reactivity with six other structurally related phthalate esters was below 1%. The recoveries of DEHP ranged from 80.8% to 119.2% indicating that the method was successfully applied for the determination of DEHP in infant supplies.