Levels of Anti-Retinal Antibodies in Retinal Detachment and Proliferative Vitreoretinopathy. (3rd June 2018)

• Record Type: Journal Article
• Authors: Ichinohasama, Reo; Nishiguchi, Koji M; Fujita, Kosuke; Aizawa, Naoko; Inoue, Takashi; Sasaki, Erika; Kunikata, Hiroshi; Nakazawa, Toru
• Is Part Of: Current eye research. Volume 43:Number 6(2018)
• Page Start: 804
• Page End: 809
• Publication Date: 2018-06-03
• Subjects: Proliferative vitreoretinopathy -- retinal detachment -- autoantibodies -- vitreous -- aqueous humor; Ophthalmology -- Periodicals; Eye -- Diseases -- Periodicals; Ophthalmology -- Periodicals; 573.88
• DOI: 10.1080/02713683.2018.1451544
• Languages: English
• ISSNs: 0271-3683

Abstract

ABSTRACT: Purpose : The purpose of the study is to investigate the correlation between intraocular anti-retinal antibodies and clinical measurements in patients with rhegmatogenous retinal detachment (RRD) and proliferative vitreoretinopathy (PVR). Material and methods : Aqueous humor and vitreous samples were collected from patients with RRD, PVR, and from control subjects with macular hole. The levels of total protein (TP), IgG, and anti-retinal antibodies were determined with a bicinchoninic acid assay, enzyme-linked immunosorbent assay, and dot blot, respectively. Correlations between these measurements were assessed using Pearson's correlation test. Analysis of variance followed by a post-hoc test or the Student t -test was used to compare differences between groups. Results : The levels of anti-retinal antibodies and IgG were correlated with each other ( P < 0.010). The IgG concentration was higher in patients with PVR than in controls in both the aqueous humor ( P < 0.001) and the vitreous ( P < 0.001), but not in patients with RRD. Conversely, TP levels and anti-retinal antibodies in both ocular fluids from RRD and PVR patients did not significantly differ from the controls. In a subgroup analysis, vitreal anti-retinal antibody levels were correlated with average macular thickness in the re-attached macula following surgery for macula-off RRD/PVR ( P = 0.012). Furthermore, patients with post-operative cystoid macular edema had a higher level of vitreal anti-retinal antibodies than those without ( P = 0.009). Conclusions : Intravitreal anti-retinal antibodies were increased in the eyes with maculopathy after surgical intervention for RRD/PVR.