Phenolic profile, antioxidant and cytotoxic properties of polar extracts from leaves and flowers of Isatis tinctoria L. (Brassicaceae) growing in Sicily. (4th July 2018)

• Record Type: Journal Article
• Authors: Taviano, M. F.; Filocamo, A.; Ragusa, S.; Cacciola, F.; Dugo, P.; Mondello, L.; Paterniti Mastrazzo, G.; De Rose, R. F.; Celano, M.; Lombardo, G. E.; Melchini, A.; Miceli, N.
• Is Part Of: Plant biosystems. Volume 152:Number 4(2018)
• Page Start: 795
• Page End: 803
• Publication Date: 2018-07-04
• Subjects: Isatis tinctoria L. -- phenolic profile -- antioxidant activity -- cytotoxicity -- human thyroid cancer cells -- Artemia salina Leach; Botany -- Periodicals; Biological systems -- Periodicals; Plants -- Periodicals; 581
• DOI: 10.1080/11263504.2017.1338629
• Languages: English
• ISSNs: 1126-3504

Abstract

Abstract: This study was designed to define and compare the antioxidant and cytotoxic properties of polar extracts obtained from basal leaves (It-BL), cauline leaves (It-CL) and flowers (It-F) of Isatis tinctoria L. growing wild around Acireale (Sicily, Italy). The phenolic profile was characterized by HPLC-PDA-ESI-MS analysis and the correlation between phenolic content and the observed biological effects was established. Further, LC/MS analysis showed that the extracts contain glucosinolates at very low concentrations. The antioxidant activity of the extracts was tested in vitro ; It-F was the most effective in the DPPH test (IC50 = 0.437 ± 0.003 mg/mL), whilst It-CL showed the best reducing power (1.546 ± 0.006 ASE/mL) and ferrous ions chelating activity (IC50 = 0.564 ± 0.011 mg/mL). The extracts exhibited anti-proliferative effects against three different human thyroid carcinoma cell lines, and It-BL displayed the strongest activity; particularly, it markedly inhibited the growth of CAL-62 cells, causing nearly 85% reduction of viability at the highest tested dose. No cytotoxicity against Artemia salina was observed. The results of our investigations indicate that the polar extracts obtained from I. tinctoria are a potential source of antioxidant and anticancer compounds, which could be suitable for nutraceutical and therapeutic applications.