Influence of zinc oxide quantum dots in the antibacterial activity and cytotoxicity of an experimental adhesive resin. (June 2018)

• Record Type: Journal Article
• Authors: Garcia, Isadora Martini; Leitune, Vicente Castelo Branco; Visioli, Fernanda; Samuel, Susana Maria Werner; Collares, Fabrício Mezzomo
• Is Part Of: Journal of dentistry. Volume 73(2018:Jun.)
• Page Start: 57
• Page End: 60
• Publication Date: 2018-06
• Subjects: Dentin bonding agents -- Polymers -- Semiconductors -- Dental caries -- Anti-bacterial agents -- Biocompatible materials testing; Dentistry -- Periodicals; Dentistry -- Periodicals; Dentisterie -- Périodiques; Electronic journals; 617.6005
• DOI: 10.1016/j.jdent.2018.04.003
• Languages: English
• ISSNs: 0300-5712

Abstract

Abstract: Objective: To evaluate the influence of zinc oxide quantum dots (ZnOQDs ) into an experimental adhesive resin regarding the antibacterial activity against Streptococcus mutans and the cytotoxicity against pulp fibroblasts. Materials and methods: ZnOQDs were synthesized by sol-gel process and were incorporated into 2-hydroxyethyl methacrylate (HEMA). An experimental adhesive resin was formulated by mixing 66.6 wt.% bisphenol A glycol dimethacrylate (BisGMA) and 33.3 wt.% HEMA with a photoinitiator system as control group. HEMA containing ZnOQDs was used for test group formulation. For the antibacterial activity assay, a direct contact inhibition evaluation was performed with biofilm of Streptococcus mutans (NCTC 10449). The cytotoxicity assay was performed by Sulforhodamine B (SRB) colorimetric assay for cell density determination using pulp fibroblasts. Data were analyzed by Student's t -test (α = 0.05). Results: The antibacterial activity assay indicated statistically significant difference between the groups (p = 0.003), with higher values of biofilm formation on the polymerized samples of control group and a reduction of more than 50% of biofilm formation on ZnOQDs group. No difference of pulp fibroblasts viability was found between the adhesives (p = 0.482). Conclusion: ZnOQDs provided antibacterial activity when doped into an experimental adhesive resin without cytotoxic effect for pulp fibroblasts. Thus, the use of ZnOQDs is a strategy to develop antibiofilm restorative polymers with non-agglomerated nanofillers. Clinical significance: ZnOQDs are non-agglomerated nanoscale fillers for dental resins and may be a strategy to reduce biofilm formation at dentin/restoration interface with no cytotoxicity for pulp fibroblasts.