Highly sensitive detection for proteins using graphene oxide-aptamer based sensors. Issue 25 (5th May 2015)

• Record Type: Journal Article
• Authors: Gao, Li; Li, Qin; Li, Raoqi; Yan, Lirong; Zhou, Yang; Chen, Keping; Shi, Haixia
• Is Part Of: Nanoscale. Volume 7:Issue 25(2015)
• Page Start: 10903
• Page End: 10907
• Publication Date: 2015-05-05
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c5nr01187f
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Polyethylene glycol (PEG)-protected GO was used to prevent the proteins using thrombin as an example from nonspecific binding. This method is a promising technique for the detection of proteins. Abstract : In recent years, the detection of proteins by using bare graphene oxide (GO) to quench the fluorescence of fluorescein-labeled aptamers has been reported. However, the proteins can be adsorbed on the surface of bare GO to prevent the sensitivity from further being improved. In order to solve this problem, polyethylene glycol (PEG)-protected GO was used to prevent the proteins using thrombin as an example from nonspecific binding. The detection limit was improved compared to bare GO under the optimized ratio of GO to PEG concentration. The results show that our method is a promising technique for the detection of proteins.