Optimization of lutein production with a two-stage mixotrophic cultivation system with Chlorella sorokiniana MB-1. (August 2018)

• Record Type: Journal Article
• Authors: Chen, Chun-Yen; Liu, Chen-Chun
• Is Part Of: Bioresource technology. Volume 262(2018)
• Page Start: 74
• Page End: 79
• Publication Date: 2018-08
• Subjects: Lutein -- Microalgae -- Mixotrophic growth -- Semi-batch culture -- Two-stage process; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.04.024
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Mixotrophic mode and semi-batch operation were preferred for the lutein production. Using the two-stage system could significantly enhance the lutein productivity. C. sorokiniana MB-1 could achieve a maximal lutein productivity of 7.62 mg/L/d. Abstract: The major purpose of this study was to investigate the effects of operational factors and bioprocess strategies on the mixotrophic cultivation of a microalgae Chlorella sorokiniana MB-1 for lutein production. Aeration with CO2 showed the highest biomass productivity and lutein productivity of 0.89 g/L/d and 3.49 mg/L/d, respectively. Semi-batch operation performed with 80% medium replacement ratio resulted in the highest biomass productivity and lutein productivity of 1.55 g/L/d and 5.51 mg/L/d, respectively. A two-stage strategy was developed to enhance the biomass production of the MB-1 strain in stage 1 with semi-batch mixotrophic culture and to optimize lutein accumulation in stage 2 under photoautotrophic conditions. The maximum biomass productivity and lutein productivity was 1.98 g/L/d and 7.62 mg/L/d, respectively, with a medium replacement ratio of 80% in stage 1. Compared with batch cultivation, the lutein productivity was enhanced by 32.7% for semi-batch operation alone and 85.9% for the semi-batch-integrated two-stage process.