Development of a novel ultrasensitive enzyme immunoassay for human glutamic acid decarboxylase 65 antibody. (July 2016)

• Record Type: Journal Article
• Authors: Numata, Satoshi; Katakami, Hideki; Inoue, Shinobu; Sawada, Hirotake; Hashida, Seiichi
• Is Part Of: Annals of clinical biochemistry. Volume 53:Number 4(2016:Jul.)
• Page Start: 495
• Page End: 503
• Publication Date: 2016-07
• Subjects: Enzyme immunoassay -- glutamic acid decarboxylase 65 -- glutamic acid decarboxylase autoantibody -- diabetes -- slowly progressive insulin-dependent diabetes mellitus; Clinical chemistry -- Periodicals; Clinical biochemistry -- Periodicals; 616.075
• DOI: 10.1177/0004563215609639
• Languages: English
• ISSNs: 0004-5632

Abstract

Background: We developed a novel, ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for determination of glutamic acid decarboxylase autoantibody concentrations in serum samples from patients with type 2 diabetes. Methods: We developed an immune complex transfer enzyme immunoassay for glutamic acid decarboxylase autoantibody and measured glutamic acid decarboxylase autoantibody from 22 patients with type 1 diabetes, 29 patients with type 2 diabetes, and 32 healthy controls. Results: A conventional ELISA kit identified 10 patients with type 1 diabetes and one patient with type 2 diabetes as glutamic acid decarboxylase autoantibody positive, whereas 15 patients with type 1 diabetes and six patients with type 2 diabetes were identified as glutamic acid decarboxylase autoantibody positive using immune complex transfer enzyme immunoassay. Conclusions: Immune complex transfer enzyme immunoassay is a highly sensitive and specific assay for glutamic acid decarboxylase autoantibody and might be clinically useful for diabetic onset prediction and early diagnosis.