Chemo-enzymatic synthesis of furfuralcohol from chestnut shell hydrolysate by a sequential acid-catalyzed dehydration under microwave and Escherichia coli CCZU-Y10 whole-cells conversion. (August 2018)

• Record Type: Journal Article
• Authors: Di, Junhua; Ma, Cuiluan; Qian, Jianghao; Liao, Xiaolong; Peng, Bo; He, Yucai
• Is Part Of: Bioresource technology. Volume 262(2018)
• Page Start: 52
• Page End: 58
• Publication Date: 2018-08
• Subjects: Chestnut shell -- Furfural -- Furfuralcohol -- SO42−/SnO2-Attapulgite -- Escherichia coli CCZU-Y10; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.04.038
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Chestnut shells (CNSs) were used to produce furfural and furfuralcohol. One-pot chemo-enzymatic catalysis was demonstrated in dibutyl phthalate-water media. Furfural was produced by SO4 2− /SnO2 -Attapulgite under microwave at 180 °C for 10 min. Bioreduction of furfural was conducted without extra addition of NAD + and glucose. Biosynthesis of furfuralcohol from furfural was performed by a fed-batch strategy. Abstract: In this study, chemo-enzymatic synthesis of furfuralcohol from biomass-derived xylose was successfully demonstrated by a sequential acid-catalyzed dehydration under microwave and whole-cells reduction. After dry dewaxed chestnut shells (CNS, 75 g/L) was acid-hydrolyzed with dilute oxalic acid (0.5 wt%) at 140 °C for 40 min, the obtained CNS-derived xylose (17.9 g/L xylose) could be converted to furfural at 78.8% yield with solid acid SO4 2− /SnO2 -Attapulgite (2.0 wt% catalyst loading) in the dibutyl phthalate-water (1:1, v:v) under microwave (600 W) at 180 °C for 10 min. In the dibutyl phthalate-water (1:1, v/v) media at 30 °C and pH 6.5, the furfural liquor (47.0 mM furfural) was biologically converted to furfuralcohol by recombinant Escherichia coli CCZU-Y10 whole-cells harboring an NADH-dependent reductase (PgCR) without extra addition of NAD + and glucose, and furfural was completely converted to furfuralcohol after 2.5 h. Clearly, this one-pot synthesis strategy can be effectively used for furfuralcohol production.