Noninvasive High-Speed Photoacoustic Tomography of Cerebral Hemodynamics in Awake-Moving Rats. Issue 8 (August 2015)

• Record Type: Journal Article
• Authors: Tang, Jianbo; Xi, Lei; Zhou, Junli; Huang, Hua; Zhang, Tao; Carney, Paul R; Jiang, Huabei
• Is Part Of: Journal of cerebral blood flow & metabolism. Volume 35:Issue 8(2015)
• Page Start: 1224
• Page End: 1232
• Publication Date: 2015-08
• Subjects: brain imaging -- functional imaging -- imaging in awake-moving animal -- neurovascular imaging -- wearable photoacoustic tomography; Cerebral circulation -- Periodicals; Brain -- Metabolism -- Periodicals; Brain -- Blood-vessels -- Periodicals; Cerebrovascular disease -- Periodicals; 612.824
• DOI: 10.1038/jcbfm.2015.138
• Languages: English
• ISSNs: 0271-678X

Abstract

We present a noninvasive method of photoacoustic tomography (PAT) for imaging cerebral hemodynamics in awake-moving rats. The wearable PAT (wPAT) system has a size of 15 mm in height and 33 mm in diameter, and a weight of ~ 8 g (excluding cabling). The wPAT achieved an imaging rate of 3.33 frames/s with a lateral resolution of 243 μ m. Animal experiments were designed to show wPAT feasibility for imaging cerebral hemodynamics on awake-moving animals. Results showed that the cerebral oxy-hemoglobin and deoxy-hemoglobin changed significantly in response to hyperoxia; and, after the injection of pentylenetetrazol (PTZ), cerebral blood volume changed faster over time and larger in amplitude for rats in awake-moving state compared with rats under anesthesia. By providing a light-weight, high-resolution technology for in vivo monitoring of cerebral hemodynamics in awake-behaving animals, it will be possible to develop a comprehensive understanding on how activity alters hemodynamics in normal and diseased states.