A capillary flow immunoassay microchip utilizing inkjet printing-based antibody immobilization onto island surfaces—toward sensitive and reproducible determination of carboxyterminal propeptide of type I procollagen. (17th March 2016)

• Record Type: Journal Article
• Authors: Fuchiwaki, Yusuke; Tanaka, Masato; Takaoka, Hiroki; Goya, Kenji
• Is Part Of: Journal of micromechanics and microengineering. Volume 26:Number 4(2016:Apr.)
• Publication Date: 2016-03-17
• Subjects: capirally -- inkjet -- immunoassay -- point-of-care-testing; Microelectromechanical systems -- Periodicals; Micromechanics -- Periodicals; 621.38105
• DOI: 10.1088/0960-1317/26/4/045015
• Languages: English
• ISSNs: 0960-1317

Abstract

Abstract: A capillary-flow-driven microchip system requires no external power and has no moving off-chip components, in contrast with most microfluidic-based immunoassay systems which are complicated to operate and require external components. To accelerate the sensitive and reproducible determination of analytes required for practical point-of-care applications, we formed island microchannel surfaces on a microcapillary channel to allow stable antibody immobilization. The island surface was surrounded by a circular groove 10 μ m deep and 150 μ m wide and allowed uniform inkjet printing of antibody spots, complete bio-reagent replacement, and sensitive detection of luminescence intensity. Quantitative analysis of carboxyterminal propeptide of type I procollagen (PICP) concentrations using this microchannel was demonstrated between 0–600 ng·ml −1, which is adequate for the clinical estimation of PICP concentrations in the blood. This microchip system holds promise as a model diagnostic platform that is readily adaptable to hands-free operation.