Utilization of Catalytic Properties of the Encapsulated Lipase with Calix[4]arene-Adorned Sporopollenin. (27th May 2018)

• Record Type: Journal Article
• Authors: Ozyilmaz, Elif; Sayin, Serkan
• Is Part Of: Polycyclic aromatic compounds. Volume 38:Number 3(2018)
• Page Start: 272
• Page End: 281
• Publication Date: 2018-05-27
• Subjects: calixarene -- enantioselectivity -- lipase -- racemic pro-drugs -- sporopollenin; Polycyclic aromatic compounds -- Periodicals; 547
• DOI: 10.1080/10406638.2016.1200638
• Languages: English
• ISSNs: 1040-6638

Abstract

ABSTRACT: The dihydrazide calix[4]arene was immobilized onto sporopollenin in order to encapsulate Candida rugosa lipase (CRL) via sol-gel entrapment. The kinetic resolution of the new encapsulated lipase was investigated for enantioselective hydrolysis of racemic naproxen methyl ester and 2-phenoxypropionic acid methyl ester. The results demonstrated that the activity and enantioselectivity of the lipase were improved when the lipase was encapsulated in the presence of calix[4]arene-immobilized sporopollenin. The encapsulated lipase showed an excellent rate of enantioselectivity against the (R/S)-naproxen methyl and (R/S)-2-phenoxypropionic acid methyl esters, with E = 350 and 295, respectively, compared to the free enzyme.