An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk. Issue 26 (18th April 2018)

• Record Type: Journal Article
• Authors: Yoshikawa, H.; Yoshinaga, M.; Tamiya, E.
• Is Part Of: RSC advances. Volume 8:Issue 26(2018)
• Page Start: 14510
• Page End: 14514
• Publication Date: 2018-04-18
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra01149d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Optical pickup ELISA with an original microfluidic disk, which enable semi-automatic sample loading and washing, was developed. The rapid and sensitive assay of C-reactive protein (CRP) was successfully performed. Abstract : We evaluated optical pickup ELISA with an original microfluidic disk that contains eight radially arranged channels, which enable semi-automatic sample loading and washing. This disk-shaped chip composed of acrylic plates was fabricated by CO2 laser machining and capture antibodies were immobilized in the channels. After the immunoreaction with antigens and enzyme-linked secondary antibodies, an enzyme-catalyzed nanoaggregation of o -phenylenediamine was detected by measuring the reflectivity change of a laser beam focused in the channel. The assay of C-reactive protein (CRP) was successfully performed in a short amount of time (approximately 20 min from CRP loading). The limit of detection was determined to be 2 ng mL −1, which is more sensitive as compared with conventional ELISA using microplates.