Characterization of sodium carboxymethyl cellulose by comprehensive two-dimensional liquid chromatography. (5th October 2015)

• Record Type: Journal Article
• Authors: Shakun, Maria; Heinze, Thomas; Radke, Wolfgang
• Is Part Of: Carbohydrate polymers. Volume 130(2015)
• Page Start: 77
• Page End: 86
• Publication Date: 2015-10-05
• Subjects: Sodium carboxymethyl cellulose -- Degree of substitution -- Size exclusion chromatography -- Gradient liquid adsorption chromatography -- Two-dimensional chromatography; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.04.011
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A two-dimensional separation method was developed for NaCMC. Simultaneous separation by DS and molar mass was achieved. Results are consistent with the assumption of DS being independent of molar mass. Mixtures composed of CMCs differing in DS and molar mass were separated. Abstract: Two series of sodium carboxymethyl celluloses (NaCMC) with average degrees of substitution (DS) ranging from 0.45 to 1.55 were synthesized from low molecular mass Avicel cellulose (Avicel samples) and from high molecular mass cotton linters (BWL samples). The samples were characterized by online two-dimensional liquid chromatography using gradient liquid adsorption chromatography in the first and size exclusion chromatography (SEC) in the second dimension. This method allows the simultaneous determination of the chemical composition (DS) and the molar mass distribution within the individual samples. Moreover information was obtained on the dependence of the elution volume in gradient chromatography on molar mass. As expected, evidence for a stronger influence of molar mass on gradient elution volume was found for the low molecular mass NaCMC as compared to the high molecular mass BWL samples. Finally the applicability of the method for the simultaneous separation of blends heterogeneous with respect to chemical composition (DS) and molar mass was demonstrated. Such blends cannot be efficiently separated by either SEC or gradient chromatography alone, nor by simply combining the results of both methods. Only the complete two-dimensional chromatogram can reveal the complexity of such blends, since it reveals the correlations between molar mass and chemical composition.