Cannabinoid receptor 2 attenuates microglial accumulation and brain injury following germinal matrix hemorrhage via ERK dephosphorylation in vivo and in vitro. (August 2015)

• Record Type: Journal Article
• Authors: Tang, Jun; Tao, Yihao; Tan, Liang; Yang, Liming; Niu, Yin; Chen, Qianwei; Yang, Yunfeng; Feng, Hua; Chen, Zhi; Zhu, Gang
• Is Part Of: Neuropharmacology. Volume 95(2015)
• Page Start: 424
• Page End: 433
• Publication Date: 2015-08
• Subjects: Neonatal stroke -- Cannabinoid receptor 2 -- Microglia -- Proliferation -- MAPK pathway; Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2015.04.028
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: Microglia accumulation plays detrimental roles in the pathology of germinal matrix hemorrhage (GMH) in the immature preterm brain. However, the underlying mechanisms remain poorly defined. Here, we investigated the effects of a cannabinoid receptor 2 (CB2R) agonist on microglia proliferation and the possible involvement of the mitogen-activated protein kinase (MAPK) family pathway in a collagenase-induced GMH rat model and in thrombin-induced rat microglia cells. We demonstrated that activation of CB2R played a key role in attenuating brain edema, neuronal degeneration, microglial accumulation and the phosphorylated extracellular signal-regulated kinase (p-ERK) protein level 24 h following GMH. In vitro, Western blot analysis and immunostaining indicated that ERK and P38 phosphorylation levels in microglia stimulated by thrombin were decreased after JWH-133 (CB2R selective agonist) treatment in a concentration-dependent manner. Microglia proliferation (EDU + microglia) and inflammatory and oxidative stress responses were attenuated by UO126 (ERK pathway inhibitor) 24 h after thrombin stimulation, an activity that was prevented by AM630 (CB2R selective antagonist). Overall, these findings suggest that activation of the endocannabinoid system might attenuate inflammation-induced secondary brain injury after GMH in rats by reducing microglia accumulation through a mechanism involving ERK dephosphorylation. Enhancing CB2R activation is a potential treatment to slow down the course of GMH in preterm newborns. Highlights: CB2R activation attenuated brain edema and neuronal degeneration following GMH in rats. JWH133 prevented ERK phosphorylation in brain tissue following GMH. ERK may regulate the proliferation and inflammatory response in thrombin induced microglia. CB2R alleviated rat brain injury following GMH partially via ERK dephosphorylation.