Chemical constituents and biological activities of Dianthus elegans var. elegans. Issue 11 (3rd June 2018)

• Record Type: Journal Article
• Authors: Mutlu, Kiymet; Sarikahya, Nazli Boke; Nalbantsoy, Ayse; Kirmizigul, Suheyla
• Is Part Of: Natural product research. Volume 32:Issue 11(2018)
• Page Start: 1245
• Page End: 1253
• Publication Date: 2018-06-03
• Subjects: Caryophyllaceae -- Dianthus elegans var. elegans -- triterpenoid saponin -- cytotoxic activity -- haemolytic activity -- immunomodulatory activity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2017.1333988
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Chemical investigation of the aerial parts of Dianthus elegans var . elegans afforded two previously undescribed saponins, named dianosides M-N (1 –2 ), together with four oleanane-type triterpenoid glycosides (3 –6 ). Their structures were elucidated as 3-O- α -L-arabinofuranosyl-16 α -hydroxyolean-12-ene-23 α, 28 β -dioic acid (1 ) and 3-O- α -L-arabinofuranosyl-(1 → 3)- β -D-glucopyranosyl 16 α -hydroxyolean-12-ene-23 α -oic acid, 28-O- β -D-glucopyranosyl-(1 → 6)- β -D-glycosyl ester (2 ) by chemical and extensive spectroscopic methods including IR, 1D, 2D NMR and HRESIMS. Both of the saponins were evaluated for their cytotoxicities against HEK-293, A-549 and HeLa human cancer cells using the MTT method. All compounds showed no substantial cytotoxic activity against tested cell lines. However, dianosides M-N and the n -butanol fraction exhibited considerable haemolysis in human erythrocyte cells. The immunomodulatory properties of dianosides M-N were also evaluated in activated whole blood cells by PMA plus ionomycin. Dianosides M-N increased IL-1 β concentration significantly whereas the n -butanol fraction slightly augmented IL-1 β secretion. All compounds did not change IL-2 and IFN- γ levels considerably. Abstract :