Circulating metabolites of strawberry mediate reductions in vascular inflammation and endothelial dysfunction in db/db mice. (15th July 2018)

• Record Type: Journal Article
• Authors: Petersen, Chrissa; Bharat, Divya; Cutler, Brett Ronald; Gholami, Samira; Denetso, Christopher; Mueller, Jennifer Ellen; Cho, Jae Min; Kim, Ji-Seok; Symons, J. David; Anandh Babu, Pon Velayutham
• Is Part Of: International journal of cardiology. Volume 263(2018)
• Page Start: 111
• Page End: 117
• Publication Date: 2018-07-15
• Subjects: BSA bovine serum albumin -- CAECs carotid artery endothelial cells -- DAF 4-amino-5-methylamino-2′, 7′-difluorofluorescein diacetate -- db/+ non-diabetic control mice -- db/db diabetic mice -- db/db + SB strawberry fed diabetic mice -- DCFDA 2′, 7′-Dichlorofluorescein diacetate -- eNOS endothelial nitric oxide synthase -- HG 25 mM glucose -- IL-8 interleukin-8 -- ICAM-1 intercellular adhesion molecule-1 -- IκKβ inhibitor κB kinase -- MAECs mouse aortic endothelial cells -- MCP1 monocyte chemoattractant protein-1 -- NFκB nuclear factor κB -- L-NAME L-NG-Nitroarginine methyl ester -- NO nitric oxide -- NOX NADPH Oxidases -- Pal 100 μM palmitate-BSA -- ROS reactive oxygen species -- TNF-α Tumor necrosis factor-α -- VCAM-1 vascular cell adhesion molecule-1; Strawberry -- Vascular inflammation -- Endothelial dysfunction -- Diabetes -- Metabolites; Cardiology -- Periodicals; Electronic journals; 616.12
• DOI: 10.1016/j.ijcard.2018.04.040
• Languages: English
• ISSNs: 0167-5273

Abstract

Abstract: Background: Cardiovascular disease is 2-4-fold more prevalent in patients with diabetes. Human studies support the cardiovascular benefits of strawberry consumption but the effects of strawberry on diabetic vasculature are unknown. We tested the hypothesis that dietary strawberry supplementation attenuates vascular inflammation and dysfunction in diabetic mice. Methods: Seven-week-old diabetic db / db mice that consumed standard diet ( db / db ) or diet supplemented with 2.35% freeze-dried strawberry ( db / db + SB) for ten weeks were compared to non-diabetic control mice ( db / + ). Indices of vascular inflammation and dysfunction were measured. Endothelial cells (ECs) were isolated from the vasculature to determine the influence of strawberry on them. The effect of metabolites of strawberry on endothelial inflammation was determined by incubating mouse aortic ECs (MAECs) with ±5% serum, obtained from strawberry fed mice (metabolites serum) or standard diet fed mice (control serum) ± 25 mM glucose and 100 μM palmitate. Results: db / db mice exhibited an increased monocyte binding to vessel, elevated blood pressure, and reduced endothelial-dependent vasorelaxation compared with db / + mice but each defect was attenuated in db / db + SB mice. The elevation of inflammatory molecules, NOX2 and inhibitor-κB kinase observed in ECs from db / db vs. db / + mice was suppressed in db / db + SB mice. Glucose and palmitate increased endothelial inflammation in MAECs but were normalized by co-incubation with metabolites serum. Conclusions: Dietary supplementation of strawberry attenuates indices of vascular inflammation and dysfunction in diabetic db / db mice. The effect of strawberry on vasculature is endothelial-dependent and possibly mediated through their circulating metabolites. Strawberry might complement conventional therapies to improve vascular complications in diabetics. Highlights: Cardiovascular disease is 2-4-fold more prevalent in patients with diabetes. Dietary strawberry attenuates vascular inflammation and dysfunction in diabetic mice. Effect of strawberry on diabetic vasculature is endothelial-dependent. Endothelial effects of strawberry are mediated through circulating metabolites. Strawberry might complement existing therapies to improve vascular complications.