Jageum-Jung improves 2, 4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in mice and suppresses pro-inflammatory chemokine production by inhibiting TNF-α/IFN-γ-induced STAT-1 and NFκB signaling in HaCaT cells. (15th July 2018) - Yang, Ju-Hye
Abstract: Ethnopharmacological relevance: Jageum-Jung (JGJ) is an oriental herbal formula comprising five herbs ( Melaphis chinensis Bell, Cremastra variabilis Nakai, Knoxia valerianoides Thorel, Euphorbia lathyris L., and Moschus moschiferus L.). It has been used for detoxification and treating cancer and inflammatory diseases in China, Japan, and Korea. However, the mechanism of action of JGJ on keratinocyte inflammatory response is poorly understood. Aim of the study: In the present study, we investigated the anti-inflammatory mechanism of JGJ and studied the effects of JGJ on atopic dermatitis-like skin lesions in mice. Materials and methods: We elucidated the anti-inflammatory and anti-inflammatory effects of JGJ on tumor necrosis factor-α/interferon-γ (TNF-α/IFN-γ)-treated human keratinocyte cells, IgE-sensitized RBL-2H3 cells, and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like mice, respectively. Results: The results showed that JGJ suppressed the production and mRNA revels of IL-8, IL-6 and, conspicuously, both TARC and RANTES. JGJ inhibited nuclear translocation of the inflammatory transcription factors NFκB and STAT-1. Moreover, JGJ improved AD-like skin lesions in DNCB-treated mice and inhibited degranulation of mast cell. Conclusions: The results of this study suggest that JGJ can be considered as a candidate agent for AD treatment. Graphical abstract: Highlights: JGJ inhibited chemokines production and mRNA expression in HaCaT cells. JGJ suppressed the activity of STAT-1, NF-kB p65 and p38 expression in HaCaT cells. JGJ suppressed translocation of NF-kB p65 and STAT-1 in HaCaT cells. JGJ improved skin conditions on DNCB-induced atopic dermatitis-like skin lesions. JGJ suppressed Ag/IgE-stimulated degranulation in RBL-2H3 mast cells.