Soil uranium concentration at Ranger Uranium Mine Land Application Areas drives changes in the bacterial community. (September 2018)

• Record Type: Journal Article
• Authors: Mumtaz, Saqib; Streten, Claire; Parry, David L.; McGuinness, Keith A.; Lu, Ping; Gibb, Karen S.
• Is Part Of: Journal of environmental radioactivity. Volume 189(2018)
• Page Start: 14
• Page End: 23
• Publication Date: 2018-09
• Subjects: Ranger Uranium Mine -- Soil bacterial community -- Uranium concentrations -- Seasonal and temporal changes -- Physicochemical parameters -- Wet-dry tropics; Radioactivity -- Periodicals; Radiation, Background -- Periodicals; Radioecology -- Periodicals; Radioactive pollution -- Periodicals; Environmental Pollutants -- Periodicals; Radioactive Pollutants -- Periodicals; Radioactivity -- Periodicals; Radioécologie -- Périodiques; Pollution radioactive -- Périodiques; Fond de rayonnement -- Périodiques; 539.752
• DOI: 10.1016/j.jenvrad.2018.03.003
• Languages: English
• ISSNs: 0265-931X

Abstract

Abstract: Soil microorganisms may respond to metal stress by a shift in the microbial community from metal sensitive to metal resistant microorganisms. We assessed the bacterial community from low (2–20 mg kg −1 ), medium (200–400 mg kg −1 ), high (500–900 mg kg −1 ) and very high (>900 mg kg −1 ) uranium soils at Ranger Uranium Mine in northern Australia through pyrosequencing. Proteobacteria (28.85%) was the most abundant phylum at these sites, followed by Actinobacteria (9.31%), Acidobacteria (7.33%), Verrucomicrobia (2.11%), Firmicutes (2.02%), Chloroflexi (1.11%), Cyanobacteria (0.93%), Planctomycetes (0.82%), Bacteroidetes (0.46%) and Candidate_division_WS3 ( Latescibacteria ) (0.21%). However, 46.79% of bacteria were unclassified. Bacteria at low U soils differed from soils with elevated uranium. Bacterial OTUs closely related to Kitasatospora spp., Sphingobacteria spp. and Rhodobium spp. were only present at higher uranium concentrations and the bacterial community also changed with seasonal and temporal changes in soil uranium and physicochemical variables. This study using next generation sequencing in association with environmental variables at a uranium mine has laid a foundation for further studies of soil-microbe-metal interactions which may be useful for developing sustainable management and rehabilitation strategies. Furthermore, bacterial species associated with higher uranium may serve as useful indicators of uranium contamination in the wet-dry tropics. Highlights: Soil bacterial community at Ranger Uranium Mine is best correlated with changes in the uranium concentration. Kitasatospora spp., Sphingobacteria spp. and Rhodobium spp. were specific to ≥200 mg kg-1 uranium concentration. New soil bacteria were recovered under unique environmental conditions of wet-dry tropics of northern Australia.