Imaging the role of toll-like receptor 4 on cell proliferation and inflammation after cerebral ischemia by positron emission tomography. Issue 4 (April 2016)

• Record Type: Journal Article
• Authors: Moraga, Ana; Gómez-Vallejo, Vanessa; Cuartero, María Isabel; Szczupak, Boguslaw; San Sebastián, Eneko; Markuerkiaga, Irati; Pradillo, Jesús M; Higuchi, Makoto; Llop, Jordi; Moro, María Ángeles; Martín, Abraham; Lizasoain, Ignacio
• Is Part Of: Journal of cerebral blood flow & metabolism. Volume 36:Issue 4(2016)
• Page Start: 702
• Page End: 708
• Publication Date: 2016-04
• Subjects: Positron emission tomography -- magnetic resonance imaging -- middle cerebral artery occlusion -- cerebral ischemia -- toll-like receptor 4 -- 3′-deoxy-3′-[18F]fluorothymidine -- [11C]PK11195 -- T2W-MRI; Cerebral circulation -- Periodicals; Brain -- Metabolism -- Periodicals; Brain -- Blood-vessels -- Periodicals; Cerebrovascular disease -- Periodicals; 612.824
• DOI: 10.1177/0271678X15627657
• Languages: English
• ISSNs: 0271-678X

Abstract

The influence of toll-like receptor 4 on neurogenesis and inflammation has been scarcely explored so far by using neuroimaging techniques. For this purpose, we performed magnetic resonance imaging and positron emission tomography with 3′-deoxy-3′-[ 18 F]fluorothymidine and [ 11 C]PK11195 at 2, 7, and 14 days following cerebral ischemia in TLR4 +/+ and TLR4 −/− mice. MRI showed similar infarction volumes in both groups. Despite this, positron emission tomography with 3′-deoxy-3′-[ 18 F]fluorothymidine and [ 11 C]PK11195 evidenced an increase of neurogenesis and a decrease of inflammation in TLR4 −/− mice after ischemia. These results evidence the versatility of neuroimaging techniques to monitor the role of toll-like receptor 4 after cerebral ischemia.