Yeast-containing feed additive alters gene expression profiles associated with innate immunity in whole blood of a rodent model. Issue 4 (May 2016)

• Record Type: Journal Article
• Authors: Branson, Jennifer A; McLean, Derek J; Forsberg, Neil E; Bobe, Gerd
• Is Part Of: Innate immunity. Volume 22:Issue 4(2016:May)
• Page Start: 249
• Page End: 256
• Publication Date: 2016-05
• Subjects: Bacterial recognition -- feed supplement -- innate immunity -- yeast cell wall; Natural immunity -- Periodicals; Endotoxins -- Periodicals; 616.07905
• DOI: 10.1177/1753425916640326
• Languages: English
• ISSNs: 1753-4259

Abstract

Feeding a yeast-containing additive (YCA; OmniGen-AF) improves immune responses in ruminant livestock and reduces subsequent production losses. The objective was to identify molecular pathways by which dietary YCA may modify immune responses using a rodent model. Thirty-seven healthy, unchallenged CD rats received a diet containing 0 (control; n = 5, only 28 d), 0.5% ( n = 15) or 1% ( n = 17) YCA for 7 ( n = 4/group), 14 ( n = 3 or 4/group), 21 ( n = 3 or 4/group) or 28 ( n = 5/group) d. At the end of the feeding periods, whole blood was collected and the isolated RNA was analyzed for the expression of 84 genes involved in innate and cell-mediated adaptive immune responses. Three bacterial pattern recognition receptors TLR1 (0.5%: + 2.01; 1%: + 2.38), TLR6 (0.5%: + 2.11; 1%: + 2.34) and NOD2 (0.5%: + 2.32; 1%: + 2.23), two APC surface receptors CD1D1 (0.5%: + 1.75; 1%: + 2.33) and CD80 (0.5%: +2.45; 1%: +3.00), and the cell signaling molecule MAPK8 (0.5%: +1.87; 1%: +2.35) were significantly up-regulated by YCA at both inclusion rates. In conclusion, feeding YCA may potentially increase recognition and responses to bacterial pathogens and T-cell activation and differentiation and thereby maintain health and prevent production losses.