Thermo-setting glass ionomer cements promote variable biological responses of human dental pulp stem cells. Issue 6 (June 2018)

• Record Type: Journal Article
• Authors: Collado-González, Mar; Pecci-Lloret, Miguel R.; Tomás-Catalá, Christopher J.; García-Bernal, David; Oñate-Sánchez, Ricardo E.; Llena, Carmen; Forner, Leopoldo; Rosa, Vinicius; Rodríguez-Lozano, Francisco J.
• Is Part Of: Dental materials. Volume 34:Issue 6(2018)
• Page Start: 932
• Page End: 943
• Publication Date: 2018-06
• Subjects: Bioactivity -- Glass ionomers -- Cytotoxicity -- Dental pulp stem cells; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2018.03.015
• Languages: English
• ISSNs: 0109-5641

Abstract

Abstract: Objective: To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells ( h DPSCs). Methods: h DPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24 h (n = 40). h DPSCs in basal growth culture medium were the control. Cell viability and cell migration assays were performed using the MTT and wound-healing assays, respectively. Also, induction of apoptosis and changes in cell phenotype were evaluated by flow cytometry. Changes in cell morphology were analysed by immunocytofluorescence staining. To evaluate cell attachment to the different materials, h DPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was determined by energy dispersive X-ray (EDX) and eluates were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with analysis of variance (ANOVA) and Student's t-test ( α < 0.05). Results: Undiluted Equia Forte extracts led to a similar cell proliferation rates than the control group from 72 h onwards. There were no significance differences between Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype. However, in presence of Equia extracts the migration capacity of h DPSCs was higher than in presence of Ionostar Molar ( p < 0.05). Also, SEM studies showed a higher degree of cell attachment when Equia Forte extracts were used. Finally, EDX analysis pointed to different weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were also detected. Significance: In summary, Equia Forte promoted better biological responses in h DPSCs than Ionostar Molar.