Whey Peptide–Based Formulas With ω-3 Fatty Acids Are Protective in Lipopolysaccharide-Mediated Sepsis. (July 2015)

• Record Type: Journal Article
• Authors: Tsutsumi, Rie; Horikawa, Yousuke T.; Kume, Katsuyoshi; Tanaka, Katsuya; Kasai, Asuka; Kadota, Takako; Tsutsumi, Yasuo M.
• Is Part Of: JPEN, Journal of parenteral and enteral nutrition. Volume 39:Number 5(2015)
• Page Start: 552
• Page End: 561
• Publication Date: 2015-07
• Subjects: whey peptide -- sepsis -- lipopolysaccharide -- antioxidants -- critical care -- enteral formulas; Parenteral feeding -- Periodicals; Enteral feeding -- Periodicals; 615.85484
• DOI: 10.1177/0148607114520993
• Languages: English
• ISSNs: 0148-6071

Abstract

Background: Sepsis and septic shock syndrome are among the leading causes of death in critically ill patients. Lipopolysaccharide (LPS) released by bacteria within the colon may translocate across a compromised epithelium, leading to oxidative stress, inflammation, sepsis, and eventually death. Methods : We examined the effects of a whey-based enteral formula high in cysteine (antioxidant precursor) and the addition of ω-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), against a mouse model of LPS-induced sepsis. Mice were fed either a whey-based diet with EPA-DHA (PAF), a whey-based diet without EPA-DHA (PSTD), or a casein-based control diet (CONT). Results : Mice fed PAF or PSTD were protected against LPS-induced weight loss. Whey-based diets suppressed inflammatory cytokine release and oxidative stress damage. Furthermore, PAF and PSTD were able to inhibit autophagy, a mechanism in which the cell recycles damaged organelles. These anti-inflammatory and antioxidative effects of PSTD and PAF resulted in decreased liver inflammation and intestinal damage and promoted protective microbiota within the intestines. Conclusions : These data suggest a clinical role for whey peptide–based diets in promoting healing and recovery in critically ill patients.