Use of a triiodide resin for isolation of axenic cultures of microalgal Nannochloropsis gaditana. (September 2015)

• Record Type: Journal Article
• Authors: Nam, Kibok; Shin, Won-Sub; Jeong, Byeong-ryool; Park, Min S.; Yang, Ji-Won; Kwon, Jong-Hee
• Is Part Of: Bioresource technology. Volume 191(2015)
• Page Start: 391
• Page End: 394
• Publication Date: 2015-09
• Subjects: Microalgae -- Triiodide resin -- Axenic culture -- Nannochloropsis gaditana; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.03.082
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Triiodide resin has a disinfectant effect on bacteria. Contaminated Nannochloropsis gaditana culture was treated with triiodide resin. TR treatment can be an effective tool for obtaining axenic microalgal culture. Growth kinetic of axenic and xenic N. gaditana was quite different. Abstract: Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a fluorescent dye SYTOX Green, three cycles of TR treatments remarkably reduced the number of viable bacteria but had little effects on the microalgae. This novel approach is a simple, rapid, and cost-effective method that can be used to isolate axenic cultures of microalgae.