Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR. (July 2015)

• Record Type: Journal Article
• Authors: Horák, Daniel; Hlídková, Helena; Trachtová, Štěpánka; Šlouf, Miroslav; Rittich, Bohuslav; Španová, Alena
• Is Part Of: European polymer journal. Volume 68(2015:Jul.)
• Page Start: 687
• Page End: 696
• Publication Date: 2015-07
• Subjects: Magnetic microspheres -- Poly(ethylene glycol) -- Real-time PCR -- DNA isolation; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2015.03.036
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: Monodisperse magnetic polymer microspheres were developed. The microspheres were coated with poly(ethylene glycol). Properties of the microspheres were characterized by a range of methods. DNA amplification in the presence of microspheres was investigated by qPCR. Optimal coating of the microspheres was found. Abstract: New monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres were synthesized, coated with poly(ethylene glycol) and thoroughly characterized using several methods, such as scanning and transmission electron microscopy equipped with energy-dispersive X-ray spectroscopy (EDX), elemental analysis, ATR FT-IR and atomic absorption spectroscopy. The effect of different coatings of the microspheres on the DNA amplification was finally investigated using a real-time polymerase chain reaction (qPCR). The particles with a relatively high density of poly(ethylene glycol) and a low amount of carboxyl groups on the surface appeared suitable for DNA isolation.