In vitro and in vivo evaluation of osteoinductivity and bone fusion ability of an activin a/BMP2 chimera (AB204): a comparison study between AB204 and rhBMP-2. (2nd November 2017)

• Record Type: Journal Article
• Authors: Zheng, Guang Bin; Lee, Jae Hyup; Jin, Yuan-Zhe
• Is Part Of: Growth factors. Volume 35:Number 6(2017:Dec.)
• Page Start: 249
• Page End: 258
• Publication Date: 2017-11-02
• Subjects: Osteoinduction -- posterolateral fusion -- activin -- AB204 -- rhBMP-2; Growth factors -- Periodicals; Physiology -- Periodicals; 611
• DOI: 10.1080/08977194.2018.1459597
• Languages: English
• ISSNs: 0897-7194

Abstract

Abstract: This study compared osteoinductivity and osteogenic capacity between AB204 and rhBMP-2 using hMSCs in vitro and a beagle's posterolateral spinal fusion model. Cultured hMSCs were treated with AB204 or rhBMP-2 with low to high doses. Three male beagles were performed posterolateral spinal fusion with biphasic calcium phosphate (2 ml) + AB204 or rhBMP-2 (20, 50 or 200 µg). They were euthanized after 8 weeks. The fusion rate and bone formation of spine samples were examined. AB204 had higher alkaline phosphatase activity, mineralization and osteogenic-related gene expression than rhBMP-2. Fusion rates in all rhBMP-2 groups were 0. They were 100% for 50 μg and 200 μg AB204 groups. Therefore, AB204 showed higher osteogenicity than rhBMP-2. It could be a better bone graft substitute.