96-Well Format-Based Microfluidic Platform for Parallel Interconnection of Multiple Multicellular Spheroids. (June 2015)

• Record Type: Journal Article
• Authors: Kim, Jin-Young; Fluri, David A.; Kelm, Jens M.; Hierlemann, Andreas; Frey, Olivier
• Other Names: Huh Dan Dongeun guest-editor.; Kim Deok-Ho guest-editor.
• Is Part Of: Journal of laboratory automation. Volume 20:Number 3(2015:Jun.)
• Page Start: 274
• Page End: 282
• Publication Date: 2015-06
• Subjects: microfluidics -- microtechnology -- lab-on-a-chip -- high-throughput screening -- automated biology; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068214564056
• Languages: English
• ISSNs: 2211-0682

Abstract

In this article, we present a microfluidic platform, compatible with conventional 96-well formats, that enables facile and parallelized culturing and testing of spherical microtissues in a standard incubator. The platform can accommodate multiple microtissues (up to 66) of different cell types, formed externally by using the hanging-drop method, and enables microtissue interconnection through microfluidic channels for continuous media perfusion or dosage of substances. The platform contains 11 separate channels, and each channel has six tissue compartments. Primary rat liver tissues were cultured over 8 days, and multiple tumor tissues (HCT116) were exposed to various concentrations of 5-fluorouracil for platform characterization.