Enhancement of immune response of piglets to PCV-2 vaccine by porcine IL-2 and fusion IL-4/6 gene entrapped in chitosan nanoparticles. (April 2018)

• Record Type: Journal Article
• Authors: Chen, Yi; Song, Tingyu; Xiao, Yong-Le; Wan, Xiaoping; Yang, Luyi; Li, Jinghai; Zeng, Guangzhi; Fang, Pengfei; Wang, Ze-Zhou; Gao, Rong
• Is Part Of: Research in veterinary science. Volume 117(2018)
• Page Start: 224
• Page End: 232
• Publication Date: 2018-04
• Subjects: Piglet -- Co-expression -- Il-2 -- Fusion IL-4/6 -- Adjuvant -- Porcine circovirus-2 vaccine; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2017.12.004
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: In order to develop a novel effective immunomodulator to enhance pig resistance against post-weaning multi systemic wasting syndrome (PMWS), a recombinant plasmid co-expressing pig interleukin-2 (IL-2) and fusion interleukin-4/6 (IL-4/6) genes, designated VRIL4/6-2, was constructed and encapsulated in chitosan (CS) nanoparticles prepared by the ionotropic gelation method. Then 21-day old piglets were divided into two groups and intramuscularly injected respectively with VPIL4/6-2-CS and saline along with the porcine circovirus-2 (PCV-2) vaccine. The blood was collected from each piglet on days 0, 7, 14, 28, 56 and 84 after vaccination to assay the immunological changes. Content of IgG2a, CD4 +, CD8 + T cells increased significantly in the sera or blood of piglets treated with VPIL4/6-2-CS (P < 0.05). Furthermore, the expression level of IL-2, IL-4, IL-6, IL-15, TLR-2, TLR-7, Bcl-2, TNF-α, CD45 and STATs (STAT1, STAT2, STAT3, STAT4) genes were significantly elevated in the treated piglets respectively in different days after inoculation (P < 0.05). The growth weight gain of the treated piglets was markedly improved in comparison with the controls (P < 0.05). These indicate that VPIL-4/6-2 entrapped with chitosan nanoparticles is a safe and promising effective adjuvant to promote the immune response of pig to PCV-2 vaccination. Highlights: A recombinant plasmid VRIL4/6-2 was constructed and encapsulated in chitosan nanoparticles. VRPIL-4/6-2-CS elevated the innate and adaptive immune response of piglets to PCV-2 vaccine. VPIL-4/6-2-CS is a promising effective adjuvant of pig to PCV-2 vaccination.