Enzyme-mediated fabrication of an oxidized chitosan hydrogel as a tissue sealant. (July 2015)

• Record Type: Journal Article
• Authors: Phuong, Nguyen Thi; Anh Ho, Viet; Hai Nguyen, Dai; Khoa, Nguyen Cuu; Quyen, Tran Ngoc; Lee, Yunki; Park, Ki Dong
• Is Part Of: Journal of bioactive and compatible polymers. Volume 30:Number 4(2015:Jul.)
• Page Start: 412
• Page End: 423
• Publication Date: 2015-07
• Subjects: Oxidized chitosan -- horseradish peroxidase -- hydrogel -- wound sealant -- injectable; Biomedical materials -- Periodicals; Polymers in medicine -- Periodicals; Polymers -- Periodicals; 547.7
• DOI: 10.1177/0883911515578760
• Languages: English
• ISSNs: 0883-9115

Abstract

Oxidized polysaccharide-based hydrogels have recently attracted much attention for tissue regeneration because of their biocompatibility and tissue-adhesive property. In this study, we introduce a new type of chitosan-based hydrogel as a tissue sealant, which was prepared by enzymatic mediation from periodate-oxidized chitosan–polyethylene glycol–tyramine. The oxidized chitosan backbone was expected to enhance the interconnection between the hydrogel layer and collagen in the tissues via the Schiff-base reaction. Proton nuclear magnetic resonance spectra indicated that tyramine-functionalized polyethylene glycol-nitrophenyl carbonate ester was conjugated to the oxidized chitosan. The degree of oxidation of the chitosan backbone was around 14% of the glucosamine units by proton nuclear magnetic resonance. The hydrogel was rapidly formed in situ (within a few seconds) in the presence of horseradish peroxidase and hydrogen peroxide. In vitro experiments with live/dead cell assays showed that the oxidized chitosan-based hydrogel was cytobiocompatible. The hydrogel exhibited high tissue adhesion strength on porcine skin models as well as good tissue-adhesive ability and wound healing properties on rabbit skin. These positive results could be promising for the application of oxidized chitosan-based hydrogels as a wound sealant.