Protein expression pattern and analysis of differentially expressed peptides during various stages of tail regeneration in Hemidactylus flaviviridis. (April 2018)

• Record Type: Journal Article
• Authors: Murawala, Hiral; Ranadive, Isha; Patel, Sonam; Desai, Isha; Balakrishnan, Suresh
• Is Part Of: Mechanisms of development. Volume 150(2018)
• Page Start: 1
• Page End: 9
• Publication Date: 2018-04
• Subjects: Epimorphic regeneration -- Lizard -- 2-DGE -- Label-free quantification; Developmental biology -- Periodicals; Molecular biology -- Periodicals; Developmental Biology -- Periodicals; Molecular Biology -- Periodicals; Biologie du développement -- Périodiques; Biologie moléculaire -- Périodiques; Developmental biology; Molecular biology; Periodicals; Electronic journals; 571.8
• DOI: 10.1016/j.mod.2018.02.001
• Languages: English
• ISSNs: 0925-4773

Abstract

Abstract: Epimorphic regeneration is a process allowing the animal to regain its lost structure which depends on the resident pluripotent stem cells as well as de-differentiation of existing cells to form multi-potent stem cells. Many studies have been done to understand the appendage regeneration mechanism. The animal model used since decades is an urodele amphibian the axolotl. However, this ability is also seen in some members of reptiles, mainly lizards which on autotomy of tail regain the same by forming a replica of its lost tail. Lizards being closer to mammals are of greater interest and cannot be neglected. Hence, a stage specific protein profiling was undertaken in order to find the peptides playing a major role in epimorphosis. 2-DGE being basic tool for creating a protein profile was used. With advent of newer modern technology, label-free analysis which uses MS/MS was also performed. The study reports the peptides involved in apoptosis, inflammation and ECM remodelling across the stages of lizard tail regeneration for the first time. Apart from these peptides, structural protein, enzymes involved in metabolism have also been highlighted in the current study to give a bigger picture of the processes and the specific peptides required for tail regeneration. Graphical abstract: Highlights: Protein profile of regenerating tail of Hemidactylus flaviviridis was generated in stage-specific manner. Level of peptides involved in various cellular processes were reported through functional characterization of label-free data. Differential expression of structural proteins and growth factors at peptide level was accounted. Representative peptides were reaffirmed by subsequent qRT-PCR and western blot analysis.