A Novel Acute Necrotizing Pancreatitis Model Induced by L-Arginine in Rats. Issue 2 (March 2015)

• Record Type: Journal Article
• Authors: Guo, Fei; Zheng, Shutao; Gao, Xiaokang; Zhang, Qiong; Liu, Jiangwei
• Is Part Of: Pancreas. Volume 44:Issue 2(2015)
• Publication Date: 2015-03
• Subjects: acute necrotizing pancreatitis -- rat -- targeted injection -- laparotomy -- L-arginine; Pancreas -- Diseases -- Periodicals; Pancreas -- Periodicals; Neuroendocrine tumors -- Periodicals; 616.37005
• DOI: 10.1097/MPA.0000000000000259
• Languages: English
• ISSNs: 0885-3177

Abstract

Abstract : Objective: This study developed a novel protocol for creating an acute necrotizing pancreatitis model in rats using L-arginine. Methods: Anesthetic laparotomy was performed on the upper abdomen, and the pancreatic parenchyma of Sprague-Dawley rats was injected with 1 mL of sterilized L-arginine solution at 5 different locations in the experimental group. Specifically, 2 different injection points in the head and body of the pancreas were chosen randomly, and 1 injection point in the tail of the pancreas was chosen randomly. The parenchyma of the pancreas was injected with 200 μL of an L-arginine solution at each point. The optimal dose of L-arginine per rat was 0.4 g/kg. Results: Serum amylase activity increased significantly after targeted injection into the parenchyma of the pancreas. Pathological examination of the pancreas 24 hours after L-arginine injection revealed massive interstitial edema, apoptosis, and necrosis of acinar cells with an infiltration of neutrophils, granulocytes, and monocytes. Conclusions: The present study developed an appropriate, workable, and reproducible rat model of acute necrotizing pancreatitis with higher survivability and success rates compared with previously published methods.