A staging table for the embryonic development of the brownbanded bamboo shark (Chiloscyllium punctatum). Issue 5 (10th March 2018)

• Record Type: Journal Article
• Authors: Onimaru, Koh; Motone, Fumio; Kiyatake, Itsuki; Nishida, Kiyonori; Kuraku, Shigehiro
• Is Part Of: Developmental dynamics. Volume 247:Issue 5(2018)
• Page Start: 712
• Page End: 723
• Publication Date: 2018-03-10
• Subjects: Shark embryonic development -- ex ovo culture -- fin development -- brownbanded bamboo shark -- evolutionary developmental biology -- staging table -- elasmobranchs; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24623
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract : Background: Studying cartilaginous fishes (chondrichthyans) has helped us understand vertebrate evolution and diversity. However, resources such as genome sequences, embryos, and detailed staging tables are limited for species within this clade. To overcome these limitations, we have focused on a species, the brownbanded bamboo shark ( Chiloscyllium punctatum ), which is a relatively common aquarium species that lays eggs continuously throughout the year. In addition, because of its relatively small genome size, this species is promising for molecular studies.Results: To enhance biological studies of cartilaginous fishes, we establish a normal staging table for the embryonic development of the brownbanded bamboo shark. Bamboo shark embryos take around 118 days to reach the hatching period at 25°C, which is approximately 1.5 times as fast as the small‐spotted catshark ( Scyliorhinus canicula ) takes. Our staging table divides the embryonic period into 38 stages. Furthermore, we found culture conditions that allow early embryos to grow in partially opened egg cases.Conclusions: In addition to the embryonic staging table, we show that bamboo shark embryos exhibit relatively fast embryonic growth and are amenable to culture, key characteristics that enhance their experimental utility. Therefore, the present study is a foundation for cartilaginous fish research. Developmental Dynamics 247:712–723, 2018 . © 2017 Wiley Periodicals, Inc. Key Findings: This study defines a staging table for the embryonic development of the brownbanded bamboo shark. This study demonstrates the experimental utility of the brownbanded bamboo shark. Culture conditions of early embryonic stages of this species are described.