Diabetes in Urethral Tissue of Female Rats: Effects of Short-Term Severe and Long-Term Mild Streptozotocin-Induced Diabetes [378]. (May 2015)

• Record Type: Journal Article
• Authors: Michelin Matheus, Selma; Marini, Gabriela; Piculo, Fernanda; Rudge, Marilza; Barbosa, Angelica Pascon; Damasceno, Debora
• Is Part Of: Obstetrics and gynecology. Volume 125(2015)Supplement 1
• Publication Date: 2015-05
• Subjects: Obstetrics -- Periodicals; Gynecology -- Periodicals; 618
• DOI: 10.1097/01.AOG.0000463731.03009.69
• Languages: English
• ISSNs: 0029-7844

Abstract

Abstract : INTRODUCTION: Recent evidence strongly suggests that urinary incontinence is a common complication among women with diabetes. In additional to these studies, we and other investigators showed that diabetes induced alterations of urethral tissue in rats. Our hypothesis was that the intensity and number of alterations on urethral striated muscle and extracellular matrix of short-term severe diabetes were higher compared with long-term mild diabetes in rats. METHODS: For the induction of mild diabetes (blood glucose between 120 and 300 mg/dL), female newborns received streptozotocin (100 mg/kg body weight, subcutaneous route); for induction of short-term severe diabetes (blood glucose level greater than 300 mg per day), adult animals received streptozotocin at 40 mg/kg, intravenous route. The rats were killed on day 133 of the experimental by intraperitoneal Thiopentax injection at 80 mg/kg and the urethrovaginal tissues were harvested. Morphometric, pathologic, and immunohistochemical to fast and slow fibers and ultrastructural analysis were conducted. RESULTS: In long-term mild diabetes were found collagen deposition, severe fibrosis, lipid droplets, and numerous subsarcolemmal and intermyofibrillar mitochondria. The effect of streptozotocin-induced short-term severe diabetes and long-term mild diabetes on urethral striated muscle and extracellular matrix of female rats resulted in diabetic myopathy in both models, which included a decreased number of fast fibers and loss of specific localization of fibers type I and type II. CONCLUSION: Long-term mild diabetes amplifies the alterations found in urethral tissue with severe fibrosis and has important implications for the monitoring and treatment strategies implemented in patients with diabetes mellitus.