Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Rabe, Alexander; Gesell Salazar, Manuela; Fuchs, Stephan; Kocher, Thomas; Völker, Uwe
• Is Part Of: Journal of oral microbiology. Volume 10:Issue 1(2018)
• Publication Date: 2018-01-01
• Subjects: Saliva -- metaproteomics -- human oral microbiome -- whole saliva proteomics -- collection method -- nLC-MS/MS; Mouth -- Microbiology -- Periodicals; Medical microbiology -- Periodicals; Mouth Diseases -- microbiology; Mouth -- microbiology; Medical microbiology; Mouth -- Microbiology; Oral Pathology; Microbiology; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.31
• DOI: 10.1080/20002297.2018.1428006
• Languages: English
• ISSNs: 2000-2297

Abstract

ABSTRACT: The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants and present a workflow including PG preparation which is suitable for metaproteomics.