Synthesis of trans-16-triazolyl-13α-methyl-17-estradiol diastereomers and the effects of structural modifications on their in vitro antiproliferative activities. Issue 150 (June 2015)

• Record Type: Journal Article
• Authors: Mernyák, Erzsébet; Kovács, Ida; Minorics, Renáta; Sere, Péter; Czégány, Dóra; Sinka, Izabella; Wölfling, János; Schneider, Gyula; Újfaludi, Zsuzsanna; Boros, Imre; Ocsovszki, Imre; Varga, Mónika; Zupkó, István
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 150(2015)
• Page Start: 123
• Page End: 134
• Publication Date: 2015-06
• Subjects: 13α-estradiol -- Azide–alkyne cycloaddition -- Antiproliferative effect -- Cell cycle blockade -- Apoptosis; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2015.04.001
• Languages: English
• ISSNs: 0960-0760

Abstract

Graphical abstract: Highlights: Novel 16-triazoles in the 13α-estrone series were synthesized via CuAAC. Some triazoles proved to be potent on a panel of human adherent cancer cell lines. One of the most potent compounds (8e ) induced cell cycle blockade in the G2/M phase. Activation of the the intrinsic pathway of apoptosis by8e was evidenced. Abstract: Novel 16-triazoles in the 13α-estrone series were synthesized via Cu(I)-catalyzed azide–alkyne cycloaddition of the two diastereomeric (on C-16 and on C-17) 16-azido-13α-estra-1, 3, 5(10)-trien-17-ol 3-benzyl ethers with substituted phenylacetylenes. The new heterocyclic derivatives were evaluated in vitro by means of MTT assays for antiproliferative activity against a panel of human adherent cancer cell lines (HeLa, MCF-7, A431, A2780, T47D, MDA-MB-231 and MDA-MB-361). The inversion of the configurations at C-16 and C-17 selectively affected the growth-inhibitory properties of the tested compounds. The 16β, 17α isomers generally proved to be potent on all cell lines, with IC50 values comparable to those of the reference agent cisplatin. Change of the substitution pattern of the phenyl group of the acetylene led to great differences in antiproliferative properties. Exclusively the p -phenyl-substituted triazoles exerted high cytostatic effects. One of the most potent compounds activated caspase-3 and caspase-9 without influencing caspase-8, confirming the induction of apoptosis via the intrinsic pathway.