Yunnanterpene G, a spiro-triterpene from the roots of Cimicifuga foetida, downregulates the expression of CD147 and MMPs in PMA differentiated THP-1 cells. Issue 27 (20th April 2018)

• Record Type: Journal Article
• Authors: Lu, Ni-Hong; Zhang, Zi-Wei; Guo, Rui-Wei; Yang, Li-Xia; Song, Ya-Xian; Ye, Jin-Shan; Shi, Yan-Kun
• Is Part Of: RSC advances. Volume 8:Issue 27(2018)
• Page Start: 15036
• Page End: 15043
• Publication Date: 2018-04-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra01895b
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Yunnanterpene G (1 ), a new cycloartane triterpene with an oxaspiro[5.4]decane moiety, was purified from the roots of Cimicifuga foetida . Significantly, it downregulates the expression of CD147 and MMPs in PMA differentiated THP-1 cells. Abstract : A new cycloartane triterpene, yunnanterpene G (1 ), containing an oxaspiro[5.4]decane moiety, was purified from the roots of Cimicifuga foetida . The new structure was determined from spectroscopic data and the X-ray diffraction method. Biological evaluations revealed that compound1 significantly inhibited the mRNA expression of the atherosclerosis-related adhesion molecule CD147 (extracellular matrix metalloproteinase inducer, EMMPRIN), and proteolytic enzymes matrix metalloproteinase 2 (MMP-2), MMP-9 and MMP-14, in a dose-dependent manner in phorbol-12-myristate-13-acetate-induced human monocytic THP-1 cells by quantitative real-time PCR method. At the same time, the migration ability of the induced THP-1 cells was potently inhibited. Furthermore, western blot experiments showed that compound1 at 25 μM strongly suppressed phosphorylation of NF-κB p65 and p38 MAPK in the differentiated THP-1 cells.