A pH-controlled bidirectionally pure DNA hydrogel: reversible self-assembly and fluorescence monitoring. Issue 36 (19th April 2018)

• Record Type: Journal Article
• Authors: Lu, Shasha; Wang, Shuang; Zhao, Jiahui; Sun, Jian; Yang, Xiurong
• Is Part Of: Chemical communications. Volume 54:Issue 36(2018)
• Page Start: 4621
• Page End: 4624
• Publication Date: 2018-04-19
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc01603h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Fluorescence monitoring using a pH-controlled reversible bidirectionally pure DNA hydrogel is reported. Abstract : A concept for a pure DNA hydrogel has been demonstrated in which triplex structures act as the core components and are assisted by single strands and artfully designed Y-shaped structures. Typically, the triplex structure which is based on protonated cytosine–guanine–cytosine (C–G·C+) is formed at pH 5.0 and disassembles at pH 7.0, whereas the thymine–adenine–thymine (T–A·T)-based triplex structure is formed at pH 7.0 and disassembles at pH 10.0. Therefore, such triplex DNA structure-based pure DNA hydrogels provide pH-controlled reversible self-assembly of DNA structures with a transition between gel and liquid states. More significantly, the introduction of both a fluorophore (FAM) and a quencher (BHQ1) to the hydrogels provides an innovative method for monitoring the self-assembly and disassembly processes through a fluorescence technology.