A narrow open tubular column for high efficiency liquid chromatographic separation. Issue 9 (10th April 2018)

• Record Type: Journal Article
• Authors: Chen, Huang; Yang, Yu; Qiao, Zhenzhen; Xiang, Piliang; Ren, Jiangtao; Meng, Yunzhu; Zhang, Kaiqi; Juan Lu, Joann; Liu, Shaorong
• Is Part Of: Analyst. Volume 143:Issue 9(2018)
• Page Start: 2008
• Page End: 2011
• Publication Date: 2018-04-10
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an02065a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow ( e.g., 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. Abstract : We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow ( e.g., 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with octadecylsilane and both isocratic and gradient separations are performed. We reveal a focusing effect that may be used to interpret the efficiency enhancement. We also demonstrate the feasibility of using this technique for separating complex peptide samples. This high-resolution and fast separation technique is promising and can lead to a powerful tool for trace sample analysis.