Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: Meyer, S.; López-Serrano, A.; Mitze, H.; Jakubowski, N.; Schwerdtle, T.
• Is Part Of: Metallomics. Volume 10:Issue 1(2018)
• Page Start: 73
• Page End: 76
• Publication Date: 2018-01-02
• Subjects: Metals -- Physiological effect -- Periodicals; 572.51
• DOI: 10.1039/c7mt00285h
• Languages: English
• ISSNs: 1756-5901

Abstract

Abstract : A fast and reliable single-cell ICP-MS/MS method is presented to determine the bioavailability of metal species as well as sulfur and phosphorus as internal control after re-suspension of cells in water. Abstract : Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0–48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods ( e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.