Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro. Issue 36 (19th April 2018)

• Record Type: Journal Article
• Authors: Chakravarthy, Madhuri; AlShamaileh, Hadi; Huang, He; Tannenberg, Rudi K.; Chen, Suxiang; Worrall, Simon; Dodd, Peter R.; Veedu, Rakesh N.
• Is Part Of: Chemical communications. Volume 54:Issue 36(2018)
• Page Start: 4593
• Page End: 4596
• Publication Date: 2018-04-19
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc02256a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We have developed a novel functional nucleic acid aptamer to amyloid-β peptide 1–40 (Aβ1–40 ) and investigated its potential to detect Aβ peptide fragments in neuropathologically confirmed Alzheimer brain hippocampus tissues samples. Abstract : We have developed a novel functional nucleic acid aptamer to amyloid-β peptide 1–40 (Aβ1–40 ) and investigated its potential to detect Aβ peptide fragments in neuropathologically confirmed Alzheimer brain hippocampus tissues samples. Our results demonstrate that the aptamer candidate RNV95 could detect tetrameric/pentameric low-molecular-weight Aβ aggregates in autopsy hippocampal tissue from two neuropathologically confirmed Alzheimer disease cases. Although these are preliminary observations, detailed investigations are under way. This is the first demonstration of aptamer-Aβ binding in Alzheimer brain tissues.