Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse. (June 2015)

• Record Type: Journal Article
• Authors: Jia, Lili; Gonçalves, Geisa A.L.; Takasugi, Yusaku; Mori, Yutaro; Noda, Shuhei; Tanaka, Tsutomu; Ichinose, Hirofumi; Kamiya, Noriho
• Is Part Of: Bioresource technology. Volume 185(2015)
• Page Start: 158
• Page End: 164
• Publication Date: 2015-06
• Subjects: Pretreatment -- Hemicellulose -- Synergism -- Endoglucanase -- Endoxylanase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.02.041
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Pretreatments resulted in different compositional and structural changes on bagasse. Presence of arabinan inhibited xylanase action on pretreated bagasse. Cellulose crystallinity and hemicellulose structure impacted on enzyme synergism. Variation of enzyme molecular structure affected the synergistic action. Abstract: The effect of pretreatment with peracetic acid (PAA) or an ionic liquid (1-ethyl-3-methylimidazolium acetate, [Emim][OAc]) on the synergism between endoglucanase and endoxylanase in the hydrolysis of bagasse was investigated. An endoglucanase, Cel6A, with a carbohydrate-binding module (CBM) and two endoxylanases, XynZ-C without a CBM and Xyn11A with an intrinsic xylan/cellulose binding module (XBM), were selected. The hemicellulose content, especially arabinan, and the cellulose crystallinity of bagasse were found to affect the cellulase–xylanase synergism. More specifically, higher synergism (above 3.4) was observed for glucan conversion, at low levels of arabinan (0.9%), during the hydrolysis of PAA pretreated bagasse. In contrast, [Emim][OAc] pretreated bagasse, showed lower cellulose crystallinity and achieved higher synergism (over 1.9) for xylan conversion. Ultimately, the combination of Cel6A and Xyn11A resulted in higher synergism for glucan conversion than the combination of Cel6A with XynZ-C, indicating the importance of the molecular architecture of enzymes for metabolic synergism.