X‐ray and UV radiation‐damage‐induced phasing using synchrotron serial crystallography. Issue 4 (12th April 2018)

• Record Type: Journal Article
• Authors: Foos, Nicolas; Seuring, Carolin; Schubert, Robin; Burkhardt, Anja; Svensson, Olof; Meents, Alke; Chapman, Henry N.; Nanao, Max H.
• Is Part Of: Acta crystallographica. Volume 74:Issue 4(2018)
• Page Start: 366
• Page End: 378
• Publication Date: 2018-04-12
• Subjects: synchrotron serial crystallography -- radiation‐damage‐induced phasing -- experimental phasing -- radiation damage -- genetic algorithms; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798318001535
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : Multi‐crystal serial crystallography data can be used for UV and X‐ray radiation‐damage‐induced phasing. Abstract : Specific radiation damage can be used to determine phases de novo from macromolecular crystals. This method is known as radiation‐damage‐induced phasing (RIP). One limitation of the method is that the dose of individual data sets must be minimized, which in turn leads to data sets with low multiplicity. A solution to this problem is to use data from multiple crystals. However, the resulting signal can be degraded by a lack of isomorphism between crystals. Here, it is shown that serial synchrotron crystallography in combination with selective merging of data sets can be used to determine high‐quality phases for insulin and thaumatin, and that the increased multiplicity can greatly enhance the success rate of the experiment.